Eppendorf FemtoJet 4x v.1 - Manual (Page 29)

Operation
FemtoJet ® 4i
English (EN)
29
6.4 Setting the compensation pressure p
c
The compensation pressure depends on the surface tension, the viscosity of the injection
liquid and the diameter of the capillary opening. The setting of the compensation pressure
must provide for a continuous slight leak of liquid at the capillary tip.
Prerequisites
• The injection parameters are known.
• Use a capillary with an opening of 0.5 μm.

Set the compensation pressure p
c
with the rotary knob.
The actual value is shown below the set value.
The set value is displayed.
6.5 Setting the injection pressure p
i
The set injection pressure must be higher than the inside pressure of the cell. The
injection pressure is built up from the start of the injection time.
Prerequisites
• The injection parameters are known.
• Use a capillary with an opening of 0.5 μm.

Set the injection pressure p
i
with the rotary knob.
6.6 Setting the injection time t
i
The injection time and the injection pressure determine the injected volume. The moment
from which the injection time is counted depends on the set synchronization mode on the
connected micromanipulator.
Beginning of time measurement during synchronization mode:
• IMMEDIATE – directly after triggering the injection
• LIMIT – when the lower safety limit is reached
Prerequisites
• The injection parameters are known.

Set the injection time t
i
with the rotary knob.
Guide values for the injection pressure are 50 hPa to 500 hPa (0.73 PSI to 7.20
PSI).
Guide values for the injection time are 0.3 – 1.5 seconds.