Eppendorf FemtoJet 4x v.1 - Manual (Page 16)

Product description
FemtoJet
® 4i
English (EN)
16
2.10.1 Compensation pressure p
c
The compensation pressure prevents the liquid from rising from the Petri dish into the
capillary due to the capillary action. Due to the compensation pressure, some liquid will
leak constantly from the capillary tip. This prevents the injection material from clumping
together.
Abb. 2-8:Pressure ratios in the capillary
Fig. 2-8: Pressure ratios in the capillary
2.10.2 Injection pressure p
i
The injection pressure defines the pressure used for injecting liquid into the cell. During
the injection process, the injection pressure is applied as long as the injection time is
running. To inject liquid into a cell, the injection pressure must be higher than the inside
pressure of the cell.
2.10.3 Injection time t
i
The injection time defines the time period for injecting the liquid. The start of the
injection time depends on the presettings of the micromanipulator. The injection time
begins either when triggering the injection function or when reaching the lower safety
limit. The injection pressure is applied as long as the injection time is running.
1 Capillary
2 Liquid with injection material
3 Compensation pressure p
c
4 Hydrostatic pressure
5 Capillary action
3
4
5
1
2