Eppendorf FemtoJet 4x v.1 - Manual (Page 27)

Operation
FemtoJet ® 4i
English (EN)
27
6.2.2 Switching off the standby mode
Prerequisites
• The display shows STANDBY .
1. Take off the injection tube.
2. Press the standby key.
The Microinjector performs a short self test.
The display shows the main screen.
6.3 Determining the injection parameters
To determine the correct injection parameters, you can carry out a test injection with
fluorescence dye.
6.3.1 Filling the capillaries with fluorescence dye
Prerequisites
• Use a capillary with an opening of 0.5 μm.
• The injection tube is mounted to the universal capillary holder.
• Pipette with Microloader by Eppendorf is available.
1. Fill the Microloader with fluorescing liquid.
2. Equip the capillary with the Microloader.
3. Insert the capillary into the universal capillary holder.
6.3.2 Carrying out a test injection
Prerequisites
• The Microinjector and the micromanipulator are connected and ready for operation.
• The universal capillary holder is prepared with a capillary and fluorescence dye.
• The universal capillary holder is clamped in the Eppendorf micromanipulator.
• The Petri dish with adherent cells is prepared.
1. Connect the injection tube to the Microinjector.
2. Define the lower safety limit on the micromanipulator.
3. Position the capillary above the cell.
4. Press the prog 1 key.
5. Press the inject key.
6. Check the injection visually.