Techne PrimeG - Manual (Page 37)

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• Enter the primer concentration in the PCR mix.
• Enter the monovalent salt concentration of the PCR
mix.
• Touch Calculate to calculate the T m
of the primer.
• To check the T m
of another primer with the same
primer and salt concentrations, touch Back and
enter the new primer sequence.
• Touch Reset to clear the entries.
• Touch Back to return to the Settings menu.
• Once all the settings and defaults have been set as required, touch Save to save the changes and
return to the Home Screen.
• If you do not wish to change any settings, touch Cancel to return to the Home Screen.