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Prime specification – Techne PrimeG User Manual

Page 17

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16

PRIME SPECIFICATION

TEMPERATURE

Block temperature range

4ºC (10°C during cycling) to 100ºC

Block uniformity (at 55ºC)

<±0.3ºC

Temperature accuracy (at 55ºC)

<±0.25ºC

Gradient availability (PrimeG only)

Between 30ºC and 80ºC

Maximum gradient (PrimeG only)

29ºC (25°C Combi-Block; 19ºC 384-well block)

Minimum gradient (PrimeG only)

1ºC

Column uniformity with a 10ºC gradient

(PrimeG only)

<±0.3ºC

Pre-run sample cooling

Yes, 4ºC

Temperature set point adjustment

0.1ºC

HEATING/COOLING RATE

Maximum heating rate

3.4ºC/s

Minimum heating rate

0.1ºC/s

Maximum cooling rate

1.0ºC/s

Minimum cooling rate

0.1ºC/s

HEATED LID

Selectable heated lid temperature

35 to 115ºC or off

Lid uniformity

±5ºC

Pre-heat lid

Yes

Warm up time from ambient

<2 min

Over-temperature cut-out

Yes

Regulated heated lid pressure

Manually adjustable

The heated lid is only operational if the block temperature is set above 35ºC

PROGRAMMING

Program interface

5.7” colour VGA LCD touch screen

Maximum number of programs stored

1000

Maximum number of stages per program

10

Maximum number of steps

10

Maximum number of cycles per stage

99

Programmable ramp rate

Yes, 0.1ºC/s steps

Maximum hold time

4h 59m 59s

Minimum hold time

1s

Gradient calculator (PrimeG only)

Yes

Incremented/decremented temperature

Yes

Incremented/decremented time

Yes

Pause facility

Yes

Program naming

Alpha numeric plus symbols

Password protection

Yes

Keyword selection

Yes

Run completion time

Yes

End of program alarm

Yes

Auto resume on power failure

Yes, always or never

Oligonucleotide T

m

calculator

Yes, based on the Nearest-Neighbour method

1

Software updates

Free of charge from

www.techne.com

1

Breslauer, K.J.; Frank, R.; Blocker, H. and Marky, L.A. (1986) Proc. Natl. Acad. Sci. USA 83, pp

3746-3750.

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