Prime specification – Techne PrimeG User Manual
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PRIME SPECIFICATION
TEMPERATURE
Block temperature range
4ºC (10°C during cycling) to 100ºC
Block uniformity (at 55ºC)
<±0.3ºC
Temperature accuracy (at 55ºC)
<±0.25ºC
Gradient availability (PrimeG only)
Between 30ºC and 80ºC
Maximum gradient (PrimeG only)
29ºC (25°C Combi-Block; 19ºC 384-well block)
Minimum gradient (PrimeG only)
1ºC
Column uniformity with a 10ºC gradient
(PrimeG only)
<±0.3ºC
Pre-run sample cooling
Yes, 4ºC
Temperature set point adjustment
0.1ºC
HEATING/COOLING RATE
Maximum heating rate
3.4ºC/s
Minimum heating rate
0.1ºC/s
Maximum cooling rate
1.0ºC/s
Minimum cooling rate
0.1ºC/s
HEATED LID
Selectable heated lid temperature
35 to 115ºC or off
Lid uniformity
±5ºC
Pre-heat lid
Yes
Warm up time from ambient
<2 min
Over-temperature cut-out
Yes
Regulated heated lid pressure
Manually adjustable
The heated lid is only operational if the block temperature is set above 35ºC
PROGRAMMING
Program interface
5.7” colour VGA LCD touch screen
Maximum number of programs stored
1000
Maximum number of stages per program
10
Maximum number of steps
10
Maximum number of cycles per stage
99
Programmable ramp rate
Yes, 0.1ºC/s steps
Maximum hold time
4h 59m 59s
Minimum hold time
1s
Gradient calculator (PrimeG only)
Yes
Incremented/decremented temperature
Yes
Incremented/decremented time
Yes
Pause facility
Yes
Program naming
Alpha numeric plus symbols
Password protection
Yes
Keyword selection
Yes
Run completion time
Yes
End of program alarm
Yes
Auto resume on power failure
Yes, always or never
Oligonucleotide T
m
calculator
Yes, based on the Nearest-Neighbour method
1
Software updates
Free of charge from
www.techne.com
1
Breslauer, K.J.; Frank, R.; Blocker, H. and Marky, L.A. (1986) Proc. Natl. Acad. Sci. USA 83, pp
3746-3750.