Luminex 200 System User Manual - Manual (Page 34)

Adjust the Sample Probe Vertical Height
Adjust the sample probe vertical height each time you change the type or style of microtiter
plate.
1. Remove the clear plastic shield that covers the sample probe area.
2. In a 96-well microtiter plate where overall height is no more than 0.75 inches (19 mm),
place the appropriate alignment tool in the plate:
• For a standard plate with flat-bottom wells - stack two of the larger (5.08 mm diameter)
alignment discs together and place them into the selected well.
• For a filter bottom plate - stack three of the larger (5.08 mm diameter) alignment discs
together and place them into the selected well.
• For a half-volume plate with flat-bottom wells - stack two of the smaller (3.35 mm
diameter) alignment discs together and place them into the selected well.
• For a round-bottom (U-bottom) plate - stack two of the smaller (3.35 mm diameter)
alignment discs in the selected well.
• For a plate with conical wells - place one alignment sphere into the selected well.
NOTE: Verify that the microtiter plate is not warped. Warped plates can lead
to incorrect probe height adjustment.
NOTE: Alignment discs can be placed in any well as long as the well is
designated in the software.
3. Eject the plate holder. Place the 96-well microtiter plate on the Luminex XYP instrument
plate holder with position A1 in the top left corner.
4. Verify that the correct well location is selected in the Luminex software, and that you are
using the appropriate number of alignment discs. Retract the plate.
5. Loosen the front thumbscrew on the probe holder one-third to one-half turn. Pull it
upward until it touches the top of the adjustment slide. Tighten the thumbscrew.
6. Use the Luminex software to lower the sample probe.
7. Loosen the front thumbscrew. Gently push the probe down until it just touches the top of
the alignment discs or sphere.
8. Tighten the front thumbscrew.
9. Raise the sample probe using the Luminex software.
10. Replace the plastic shield that covers the sample probe area.
After Running Samples
1. Sanitize with a 10% to 20% household bleach solution.
2. Run two Wash cycles with distilled water.
3. Soak with distilled water. Wait until the soak completes.
4. If desired, turn off the Luminex 200 analyzer.
Routine Tasks
Luminex ® 200 ™ System User Manual
22
For In Vitro Diagnostic Use