Ocean Optics OOIIrrad-C - Manual (Page 59)

5: Calibration
a. While in Scope mode, adjust the data acquisition parameters in the Nom. Integ. Period field
for a signal maximum at around 3500 counts when the calibration lamp is on.
b. Use a boxcar smoothing width (Smoothing Size) of about 5, unless calibration is intended for
use with “spikey” sources.
c. Use the single scan mode and keep the number of averages (Scans to Avg) at 1 until you
store a reference spectrum. At that point, you should increase the setting to 100. This may
slow your response time, but it will improve the calibration results.
3. Click the Store Dark button. The Store a scan screen for a dark scan appears. Follow the
directions on the screen.
Note
The Subtract Dark checkbox on the OOIColor/OOIIrrad screen is enabled by default
when in Scope mode and affects Scope mode only. A dark spectrum is used in the
calculations of all other modes by default.
4. Turn on the lamp and let it warm up for at least 20 minutes.
5. On the
OOIColor/OOIIrrad screen, click the Ref button to store a reference scan of the lamp.
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