Ocean Optics OOIIrrad-C - Manual (Page 58)

5: Calibration
A new trace (thick) will be overlayed. By default this trace will be layed over the VIS calibration
trace and then over the UV trace. If the UV calibration trace value at 400nm is different than the
VIS calibration at 400nm, the UV calibration will be multiplied by the factor of these two values
so one continuous calibration curve is achieved. You can change the priority from VIS calibraiton
to UV calibration using the Spectrometer | UV Range Priority menu item.
6. Select
Windows | Autoscale from the menu bar. Some manual scale adjustment might also be
required.
7. Select File | Save UV/VIS Calibration from the menu bar. A prompt appears for the file name
and path.
8. Label the calibration file with spectrometer’s serial number and _UV_VIS. The extension .cal is
added automatically and this file name displays in window.
9. Select File | Print from the menu bar to print the file. Include all three spectrometer calibration
files (if applicable).
10. Close this window and go back to the Irradiance Calibration screen.
11. Select Spectrometer | Active Spectrometer Calibration File from the menu bar. You are then
prompted to select and activate a spectrometer calibration file. Select the newly created UV-VIS
calibration file as the spectrometer’s active irradiance calibration file.
Calibrating Relative Irradiance
The Relative Irradiance measurement requires a lamp with known color temperature (in degrees Kelvin)
to calibrate the spectrometer for irradiance readings, which is the correct shape on a scale of 0 to 1. This
known shape is defined for many lamps as color temperature. When a reference spectrum is stored for a
lamp with a known color temperature, the OOIIrrad-C application creates an internal conversion
(calibration) to compensate for the spectrometer’s efficiency and sensitivity issues. The result is a correct
spectrum (in shape) on a sale from 0 to 1.
► Procedure
1. Set up the optical path from the lamp to the spectrometer. Be sure to include all optional
components such as fibers, integrating sphere, and cosine corrector.
2. Use the
OOIColor/OOOIIrrad main screen to set the following:
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