Bio-Rad BioLogic LP System User Manual

Page 88

5.4.2.2 Holding a Run

Runs can be placed on Hold manually by pressing the Hold softkey on the Run Screen or they may occur
automatically when an Alarm is programmed with Hold On. For information on programming Alarms with
Hold On see section 5.3.6.

Table 25.

Run Mode: Run Hold

Reference

Description

During a Hold, all instruments continue to operate as they were
when the Hold took effect; however the elapsed time or volume
in the run stops changing. The pump continues to deliver the
same buffer even if the Hold is placed in the middle of a gradi-
ent. For example if a 0-50%B gradient was placed on Hold at
33%B, then 33%B will continue to be delivered. If fractions
were being collected, then they continue to be collected with the
same fraction size and fraction advances. The Continue soft-
key concludes the Hold by starting the elapsed time or volume
counter.

Note: Since the elapsed time or volume is not increasing during
a Hold, the run will go on indefinitely. This can cause buffer
containers to become empty, fraction collectors to run out of
tubes, and other problems. This feature must be used carefully.

System Operation

R2b

Method: <>

Volume: 0.51 ml

Pump: 1.00 ml/min A

UV:

0.0208 AU

Frac: Collecting

Cond:

0.10 mS

CONTINUE

STOP RUN

(confirm and exit

Run mode)