Bio-Rad BioLogic LP System - Manual (Page 32)

2. Pull the platen cam lever away from the pump head to unlock the platen and slide the platen away from
the pump head frame assembly, exposing the rollers.
3. Slip a lock-ring onto one end of the tubing. The lock rings must be put on before luer fittings, and the
recessed parts of the lock rings should face each other. The size and color of each lock ring is as fol-
lows:
Tubing ID Lock Ring Color
0.8 mm (1/32”) Red
1.6 mm (1/16”) Orange
3.2 mm (1/8”) Yellow
Insert a barbed female luer-fitting into the same end until the tubing reaches the flange of the fitting.
Clamp the luer fitting into place by sliding the lock-ring along the tubing over the barbed fitting. Repeat
the procedure for the other end of the tubing. For flow rates greater than 20.0 ml/min see the note
below.
4. Insert one end’s fitting into one of the tubing retaining brackets of the pump head. Pull the tubing
around the rollers, and insert the other end’s fitting into the bracket on the opposite side of the pump
head. Use the same bracket on each side of the pumps, either the front brackets or the back brackets.
5. Slide the platen back into the pump head frame assembly until it contacts the tubing. Press the cam
lever in toward the pump head, locking the platen up against the tubing and rollers. Note that the platen
can be inserted with the cam lever on the left or the right.
Note: To obtain flow rates from 20.0 to 40.0 ml/min prepare two pieces of 3.2 mm pump tubing as
in step 3. Use two Female “T” Connectors, see figure 12, and four short lengths of tubing with male
luer fittings on both ends to join the two pieces together. Place both pieces of pump tubing into the
pump head as in step 4. For accurate flow rates when this is done you must perform User
Calibration. Refer to section 6.2.2.
Caution
Each time the pump’s tubing is changed, especially if the tubing size is changed,
the pump must be recalibrated. Refer to Section 6.2, Pump Calibration.
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System Plumbing
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