Bio-Rad BioLogic LP System - Manual (Page 49)

5.2.4 Manual Mode Operation of the UV Monitor
Table 9 discusses each of the functions available in Manual mode operation of the UV Monitor. For an
overview of these functions, refer to Figure 18.
Table 9.
Manual Mode Operation: UV Monitor
Reference Description
To manually control the UV Monitor:
a.
From the Mode keys select Manual.
b. From the Instrument keys select UV.
c. The main menu is shown below.
The display shows the following:
•
UV Monitor: The current UV reading.
• Range/Max Range: The chart range for UV detection.
Description of the soft key choices follows.
Set Range: Allows you to increase/decrease the full-scale AU
(Absorbance Unit) value for the UV output to the chart recorder.
For example, when the range is set to 1.000 AUFS
(Absorbance Units Full Scale), the chart recorder pen will be at
its highest point (“full scale”) when the absorbance of the sam-
ple in the flow cell is 1 AU. The following set ranges are avail-
able: 2.0, 1.0, 0.5, 0.2, 0.1, 0.05, 0.02, 0.01, 0.005, 0.002,
0.001 AUFS.
Zero: This allows you to zero the UV baseline on the chart
recorder.
42
System Operation
Method: <> Manual Operation
UV Monitor: 0.0111 AU
Range: 1.000 AUFS
SET RANGE ZERO SET MAX LAMP
MU
M3 M4 M1 M2
Method: <> Manual Operation
UV Monitor: 0.0111 AU
Range: 1.000 AUFS
SET RANGE ZERO SET MAX LAMP
M2
M3 M4 M1
Method: <> Manual Operation
UV Monitor: 0.0111 AU
Range: 1.000 AUFS
INCREASE DECREASE CANCEL OK
M1
MU
MU
(Increment/decrement
the range by
approximately 50%
each time the button
is pressed)
(sets the
current UV
reading to
0.0000)
Manual UV