Bio-Rad BioLogic LP System - Manual (Page 68)

61
System Operation
Method: Volume: 1.00 ml
Step: 1 Buffers: G M [A] B C D E
(GRAD=Linear Gradient, MIX=%B Mixture)
GRAD
MIX CANCEL OK
Method: Volume: 1.00 ml
Step: 1 Isocratic Mixture Conditions
Mixture: _ %B
CANCEL OK
Method: Volume: 1.00 ml
Step: 1 Linear Gradient Conditions
Initial: 0%B Final: 0%B
(initial)
FINAL CANCEL OK
Method: Volume: 1.00 ml
Step: 1
Length of Step: 0.00 ml
CANCEL OK
Method: Volume: 1.00 ml
Step: 1
Flow Rate: 1.00 ml/min
CANCEL OK
SEE SECTIONS
5.3.5 - 5.3.6
SEE SECTIONS
5.3.5 - 5.3.6
PT
P2
P1
P2a
P2b
P3
P4
PT
return
to P1
return
to P1
return
to P1
return
to P1
return
to PT
P1 PUMP TABLE
LENGTH OF STEP P3
FLOW RATE FOR THE STEP P4
SELECT BUFFER/SOLUTION FOR THE STEP P2
PROGRAM A LINEAR GRADIENT USING BUFFERS A & B P2a
PROGRAM A MIXTURE OF BUFFERS A & B P2b
LEGEND
Step Vol(ml) Buffer ml/min Total (ml)
> 01 10.00 Buffer A 1.00 1.00
02 New Step to be added
INSERT EDIT DELETE OK
Step Vol(ml) Buffer ml/min Total (ml)
01 10.00 Buffer A 1.00 1.00
> 02 New Step to be added
ADD OK
P1
Figure 24. Programming: the Pump Table
(Screen displays are keyed to the discussion in Table 16)