Bio-Rad BioLogic LP System - Manual (Page 7)

3. Make final adjustments. From the Mode keys select Manual, and from the Instrument keys select
Pump. Start the pump, and with fluid flowing through the system, check the following:
a. Check the range setting for the UV Monitor, zero the UV Monitor, and position the Channel 1 pen on
the chart recorder.
b. Check the range setting for the Conductivity Monitor, and position the Channel 2 pen on the chart
recorder.
c. Check that a sufficient number of tubes are in the fraction collector rack.
d. Check all fittings for leaks.
e. Turn the MV-6 valve know to the left, and load the sample loop.
f. Check that the “Waste” tube is in the waste container or drain, and make sure that buffer inlet lines
are submerged to the bottom of their containers.
4. Run the method: From the Mode keys select Run to start the separation. When the run starts, turn the
MV-6 Inject valve knob to the right. (Refer to section 5.4.)
Getting Started