Glossary – Bio-Rad Bio-Plex Data Pro™ Software User Manual
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Bio-Plex Data Pro Software User Guide
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Glossary
Analyte
The substance being measured, identified, quantitated, or otherwise analyzed 
in an experiment. In the Bio-Plex® system, analytes are molecules that are 
being quantitated.
Attribute
Custom field relevant to customer research, entered manually or copied in 
from another list (Excel, for example).
Concentration
The amount of substance present in a sample, usually expressed in weight 
per volume.
Data set
The sample and analyte information available after a run has been performed 
and analyzed. A data set is roughly equivalent to a plate and is imported via a 
file import; for example, from Bio-Plex Manager™ software via an RBX file.
FI
Fluorescent intensity. A relative measurement of fluorescent intensity based 
on the median, a robust statistical measure.
Heat map
Graphical representation of peak expression data. Each column of a heat map 
represents a spectrum, each row represents a peak cluster, and each cell 
represents a specific peak that is color coded to reflect its expression level. 
Displays relationships between samples and analytes.
LLOQ
Lower limit of quantitation.
LOD
Limit of detection.
Outlier
Sample concentration derived from FI below the background value. Sample 
concentration that meets a user-defined criteria, including %CV, 
concentration above or below a certain range.
