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Glossary – Bio-Rad Bio-Plex Data Pro™ Software User Manual

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Bio-Plex Data Pro Software User Guide

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Glossary

Analyte

The substance being measured, identified, quantitated, or otherwise analyzed
in an experiment. In the Bio-Plex® system, analytes are molecules that are
being quantitated.

Attribute

Custom field relevant to customer research, entered manually or copied in
from another list (Excel, for example).

Concentration

The amount of substance present in a sample, usually expressed in weight
per volume.

Data set

The sample and analyte information available after a run has been performed
and analyzed. A data set is roughly equivalent to a plate and is imported via a
file import; for example, from Bio-Plex Manager™ software via an RBX file.

FI

Fluorescent intensity. A relative measurement of fluorescent intensity based
on the median, a robust statistical measure.

Heat map

Graphical representation of peak expression data. Each column of a heat map
represents a spectrum, each row represents a peak cluster, and each cell
represents a specific peak that is color coded to reflect its expression level.
Displays relationships between samples and analytes.

LLOQ

Lower limit of quantitation.

LOD

Limit of detection.

Outlier

Sample concentration derived from FI below the background value. Sample
concentration that meets a user-defined criteria, including %CV,
concentration above or below a certain range.