Bio-Rad Image Lab™ Software - Manual (Page 81)

Creating a Protocol
User Guide | 81
The selected channel is used as the normalization factor against which all other
lanes in the channels are compared to normalize the volume data.
You can defer making a selection during the protocol setup and normalize your
data after the image is generated. For more information on normalizing volume
data, see Normalizing Volume Data on page 143 .
Note: You must select an application for at least one channel in Step 1.
Gel Imaging for Multichannel Protocols on page 74 in order to see a list of
choices.
2. Select the type of normalization to use:
 Total Lane Protein Normalization — one lane in the normalization
channel is used to calculate the normalization factor.
 Housekeeping Protein Bands Normalization — a single band of a
housekeeping protein is used to calculate the normalization factor.