Bio-Rad Image Lab™ Software - Manual (Page 150)

6 | Analyzing Images
150 | ChemiDoc MP Imaging System with Image Lab Software
 Lanes contain saturated pixels — check your images for saturated
pixels, indicated in red. These points cannot be read and cannot be used in
quantification.
 Poor transfer quality — check the quality of the transfer. If the transfer is
poor with splotchy or blurred areas, delete these lanes.
View the Data in the Analysis Table
The first nonstandard lane in the selected channel is used as the normalization
factor against which all other lanes in all the channels are compared. The
normalization values are calculated based on the total background-corrected signal
in the selected channel.
Saturated Pixels