Bio-Rad Components for Older Model Spot Cutter - Manual (Page 3)

Table of Contents
Page
Section 1 Introduction ..................................................................................................1
1.1 General Description....................................................................................................1
1.2 Components Checklist ...............................................................................................1
1.3 Caution........................................................................................................................1
1.4
Moving The Spot Cutter.............................................................................................1
1.5 Computer Recommendations.....................................................................................1
1.6 Cutting Excision Sizes ...............................................................................................1
1.7 Power Setting .............................................................................................................1
Section 2 Getting Started .............................................................................................2
2.1 Software Description..................................................................................................2
2.2 Installing Camera Driver (PVCAM 2.5.8) and the PCI Card ...................................3
2.3 Installing PDQuest Software......................................................................................4
2.4 Spot Cutter Setup........................................................................................................5
2.5 System Description ....................................................................................................5
2.6 Camera Mount Installation.........................................................................................7
2.7 Camera Installation ....................................................................................................7
2.8 System Startup............................................................................................................8
2.9 Power “ON” Startup Sequence ..................................................................................8
2.10 Software Features.......................................................................................................9
Section 3 Calibration..................................................................................................14
3.1 Camera Calibration: Lens Aperture Setting ............................................................14
3.2 Camera Calibration: Focus.......................................................................................15
3.3 Camera Alignment—Rotation & Centering............................................................15
3.3.1
Rotation......................................................................................................15
3.3.2 Centering....................................................................................................16
3.3.3 Switch to White Light Imaging .................................................................16
3.4 Camera Alignment—X Crop...................................................................................17
3.5 Setting Cutting Tip Height.......................................................................................17
3.6 Calibration of the Microtiter Plate (MTP) ...............................................................18
3.6.1
Calibration of MTP A1 Position................................................................18
3.6.2 Assignment of Microtiter Plate Deposit Sequence ...................................19
3.7 Calibration of the Wash Well Position ....................................................................19
3.8 X, Y Calibration of Cutting Platform ......................................................................21
3.8.1
Prepare Coomassie Stained PVDF Membrane .........................................21
3.8.2 Calibration of X, Y Coordinates................................................................21
Section 4 Spot Excision ..............................................................................................23
4.1 Gel and Blot Preparation..........................................................................................23
4.2 Transfer of Gel or Blot to Cutting Platform ............................................................23
4.3 Set-up PDQuest Basic Exicision Software for Spot Excision ................................23
4.4 Starting the Spot Excision Process ..........................................................................26
Section 5 Maintenance and Adjustments.................................................................26
Section 6 Troubleshooting..........................................................................................29
Appendix A System Box (Front) ....................................................................................34
Appendix B Working Surface ........................................................................................35
Appendix C System Box (Back) .....................................................................................36
Glossary.......................................................................................................37
Systems Record ..........................................................................................38