22 separation column – Metrohm 850 Professional IC Anion MSM-HC - LP-Gradient User Manual
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3.22 Separation column
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850 Professional IC Anion MSM-HC LP Gradient
￭
Remove locking caps and/or stoppers from the guard column.
￭
Fasten the lower end of the guard column with a 6.2744.070
short PEEK pressure screw to the column input capillary .
￭
Fasten the connection capillary (3.4224.240) enclosed with the
guard column to the upper end of the guard column with a
6.2744.070 short PEEK pressure screw.
Guard columns are also available which can be screwed with the
upper end directly onto the separation column.
2 Rinsing the guard column
￭
Place beaker under the outlet capillary of the guard column.
￭
Start the high pressure pump and rinse the guard column approx.
5 minutes with eluent. Set the flow according to the correspond-
ing column information sheet.
￭
Switch off the high pressure pump again.
3.22
Separation column
The intelligent separation column (iColumn) is the heart of the ion chro-
matographic analysis. It separates the different components correspond-
ing to their interactions with the column. Metrohm analytical columns are
equipped with a chip on which their technical specifications and their his-
tory (first use / setting up, operating hours, injections, ... ) are saved.
Note
Information regarding which separation column is suitable for your
application can be found in the Metrohm IC Column Program, the
in the product area ion chromatography, or
obtained directly from your agent.
Caution
New separation columns are filled with solution and are sealed on both
sides with stoppers. Before using the column, you need to ensure that
this solution is miscible with the eluents used (observe manufacturer's
data).
You can find the separation columns and guard columns currently availa-
ble from Metrohm in the Metrohm IC Column Program, or in the Internet
test chromatogram and an information sheet are provided along with