Metrohm 850 Professional IC Anion MSM-HC - LP-Gradient User Manual
Page 109
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6 Troubleshooting
850 Professional IC Anion MSM-HC LP Gradient
￭￭￭￭￭￭￭￭
99
Problem
Cause
Remedy
Individual peaks
greater than expec-
ted
Sample – Carry-over of the
samples from previous
measurement.
Rinse system longer between two samples.
MSM-HC – No (or
insufficient) pump-
ing of regeneration
or rinsing solution
Leak in the system.
Check connections.
Peristaltic pump – contact
pressure too weak.
Correctly set contact pressure .
Peristaltic pump – filter
blocked .
Replace the filter .
MSM-HC – backpressure
too high.
Clean the MSM-HC (see Chapter 5.11.3.2,
page 87) or replace parts (see Chapter
5.11.3.3, page 90).
Peristaltic pump – pump
tubing defective.
Replace pump tubing .
Poor reproducibility
of the retention
times
Eluent – Leak in the eluent
path.
Check the eluent path
Eluent – Blockage in the
eluent path.
Check the eluent path
Chromatograms
have poor resolution
Separation column –
diminished separating effi-
ciency.
￭
Regenerate separation column (see Chap-
ter 5.13.4, page 95).
￭
Replace separation column (see "Connect-
ing and rinsing the separation column",
page 63).
Extreme spread of
the peaks in the
chromatogram.
Splitting (dual
peaks)
Connections – dead vol-
ume in the system.
Check connections (see Chapter 3.6, page 18)
(use PEEK capillaries with an internal diameter
of 0.25 mm between the injection valve and
detector).
Guard column – diminished
performance.
￭
Replace guard column (see Chapter 3.21,
page 61).
Separation column – dead
volume at column head.
￭
Install separation column in reverse flow
direction (if permitted by the information
sheet).
￭
Replace separation column (see "Connect-
ing and rinsing the separation column",
page 63).
Greater rise in the
baseline
MSM-HC – reduced
capacity.