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3 staining in two rows, 1 doubling the specimen throughput, Staining in two rows – Leica Biosystems ST4040 User Manual

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Leica ST4040 – Linear Stainer

6.

Operation

6.3

Staining in two rows

6.3.1 Doubling the specimen throughput

For this type of application, the sequences of reagent / tap water sta-
tions in rows 1 and 2 must be identical.

According to the station sequence selected for row 1 (see Chapter 6.2)
insert an identical sequence in row 2.

Both rows are run with the same program.

6.3.2 Carrying out two different, but matching, staining protocols simultaneously

Precondition: the program parameters of both protocols must be the
same (i.e. both rows are run with the same program).

The two staining protocols are coordinated through the number of re-
agent stations and/or the concentration or dilution of the reagents (for
details, see Appendix 1

- ‘Staining protocols’).

Important note to chapters 6.3.1 and 6.3.2

When staining in two rows, standard slide rack carriers, as for single
row staining, are used.

Slide rack carriers located side by side in rows 1 and 2 are inserted
laterally reversed into the same notch of the conveyor frame (see left).

6.3.3 Carrying out two different staining protocols (two subsequent single loads)

The two rows are never carried out simultaneously, i.e.: row 1 is run
with program 1 - row 2 with program 2, or vice versa.

In 6.3.3 the advantage of staining in two rows is that 2 different staining protocols, though not
run simultaneously, can be run one directly after another without any delay; whereas in a sin-
gle load model, stations would have to be rearranged, refilled etc. prior to being able to run the
second protocol.