Leica Biosystems ST4040 - Manual (Page 49)

49 Leica ST4040 – Linear Stainer
Appendix 1
Staining Protocols for Leica ST4040 Linear Stainer
Xylene
Xylene
Xylene
Xylene
100 % Alcohol
100 % Alcohol
96 % Alcohol
75 % Alcohol
Running water
Distilled water
Haemalum
Haemalum
Running water
Running water
25 % HCl in water
Running water
75 % Alcohol
Eosine
Eosine
96 % Alcohol
96 % Alcohol
100 % Alcohol
100 % Alcohol
100 % Alcohol
Xylene
Xylene
Xylene
Xylene
Xylene
Xylene
100 % Alcohol
96 % Alcohol
75 % Alcohol
Resorcinol G
Resorcinol G
Running water
Running water
Distilled water
Weigert iron haemalum
Weigert iron haemalum
25 % HCl in 96 % alcohol
Running water
Running water
Distilled water
van Gieson
van Gieson
96 % Alcohol
96 % Alcohol
100 % Alcohol
100 % Alcohol
100 % Alcohol
Xylene
Xylene
Xylene
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
Elastica van Gieson Station no. H & E
Immersion time: 1 Minute
Draining time: 5 Seconds
Agitation: ON