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2 inserting the knife holder, Inserting the knife holder, Operation – Leica Biosystems RM2245 User Manual

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Instructions for Use V 1.9 RevC - 06/2013

Setting up the knife holder base
• Release the clamping lever (50) by rotating it

counterclockwise.

• Insert the knife holder base (51) using the

notch (52) on the bottom into the T-piece (55)

of the microtome base plate (53).

• To secure the knife holder base, turn the

clamping lever (50) clockwise.

The knife holder base (51) can be moved back and

forth on the microtome base plate. This allows

bringing the knife holder to optimal sectioning

position in relation to the specimen.
There is a scale (54) on the right side of the micro-

tome base plate. This enables faster and better

positioning of the knife holder at the specimen

if various combinations of standard specimens

and specimen holders are used. The rear edge of

the knife holder base (51) functions as the scale

reference.

55

50

51

52

53

5.2 Inserting the knife holder

Inserting the knife holder
• Loosen the screw (58) using an Allen key size 4

(71) until the knife holder (57) can be moved.

• Place the knife holder (57) with the underside

groove onto the T-piece (56) of the knife holder

base (51).

• To clamp, retighten the screw (58).

Enlarged detail:

Scale for better

repositioning of

the knife holder for

varying specimen

heights.

54

5. Operation

56

57

58

71

Fig. 22

Fig. 23