Operation – Leica Biosystems RM2245 User Manual

Page 26

Instructions for Use V 1.9 RevC - 06/2013

5. Operation

Multiple

step

backward

Multiple

step

forward

Single

step

backward

Single

step

forward

Sectioning mode

Button functions in STEP

mode

In sectioning mode the user can select between STEP function (step-

by-step specimen feed) and continuous specimen feed.

This feature is only available in sectioning mode in version 2.1 or

higher. For software versions older than 2.1, contact Leica Technical

Service.

When continuous feed is selected, the coarse feed buttons have the same func-

tions as in trimming mode. The STEP function is useful for careful step-by-step
approximation of the specimen towards the blade.

How to activate the STEP function:

•

Switch the instrument on while holding the

button on the control panel.

(Likewise, to deactivate switch the instrument on while pressing the

button.)

While the instrument is initializing, hold the

button until the software version

number is no longer displayed (ensure that version number 2.1 was displayed).

• Press the TRIM/SECT

button and select sectioning mode (LED SECT lit).

• When pressing the button (with an arrow) for slow-speed coarse feed, a defined,

incremental feed takes place (STEP) at the value indicated on the display in the

respective direction (simple step).

• By short activation of the quick coarse feed buttons, a single step is also effected

in the appropriate direction.

• Longer activation of the double-arrow coarse feed button effects a repeated

feed motion for as long as the button is pressed.

Backwards coarse feed

Trimming mode

• To start the rapid backwards movement (away from the blade) press the

key.

After the

button is pressed, the specimen head is moved to the rear end

position.

• To stop the movement, press any of the four coarse feed buttons.

• The yellow LED (40) in the button flashes while the specimen head is in mo-

tion, and remains lit continuously when the rear end position is reached.

In the trimming mode, the coarse feed buttons operate a continu-

ous movement as long as the button is held down. The double-arrow

button for rapid coarse feed backward movements has a lock-in

function.
Do not put your fingers between the specimen clamp and microtome

to prevent pinching them.