2 trimming, Trimming, Operation – Leica Biosystems SM2500 User Manual
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Leica SM2500
5.6.2 Trimming
Trimming can be done in modes of operation AUTO or PROG.
Trimming in AUTO mode of operation:
Preparatory steps:
•
Activate AUTO mode of operation (see chapter 5.4.3).
•
Select the desired section thickness (see chapter 5.4.5).
•
Activate retraction / select retraction setting as needed (chapter 5.4.5).
•
Select sectioning / milling and return stroke speed (see chapter 5.4.7).
•
Select a sectioning mode (see chapter 5.4.6) (for trimming usually
CONT).
•
Turn the knife guard away from the knife edge.
Start the trimming procedure:
Press RUN STOP + RUN ENABLE (see chapter 5.4.10) or press the foot
switch (see chapter 5.4.1).
•
Trim the specimen down to the desired level.
•
Press RUN STOP or RUN ENABLE or once again press the foot switch to
stop trimming.
Trimming in PROG mode of operation
Preparatory steps:
•
Activate PROG mode of operation.
The difference between PROG and AUTO is that in PROG none of
the three sectioning modes (area 2.3, chapter 5.4.6) is selected, but
a programmed number of sections is carried out.
•
Select section thickness, retraction (if needed) and speed as described
for AUTO mode of operation.
•
Select the desired number of sections ‘n’ you wish to be carried out
(area 2.2 chapter 5.4.5).
Start the trimming procedure:
•
Press RUN STOP + RUN ENABLE or press the foot switch:
the instrument carries out the preselected number of sections and then
stops in the selected stop or photo position.
5.
Operation