Leica Biosystems SM2500 - Manual (Page 58)

58 Instruction manual V 1.3 – 10/2007
5.5.12 Working with fixed-setting (45°) declination blocks and knife holder B
Steps 1 - 4 for all knife lengths:
1. Place left declination block (1) (with clearance angle scale (5)) onto left
microtome bearing block and secure with 3 screws (6) (Allen key size 8).
The bearing surfaces must be clean. Place declination blocks onto
bearing blocks from above - do not slide them onto the bearing
blocks to prevent the bearing surfaces from getting scratched.
2. Insert the left cylindrical holder (3) (marked by a black line) from the in-
side outwards through declination block (1) until the left locking pin (10)
is released.
3. Pull the left cylindrical holder (3) outwards as far as it will go and turn it
upwards until clamping slot (12) is located at the top; secure with Allen
screw (8) using an Allen key size 8.
4. Place the right declination block (2) onto the right bearing block of the
microtome and fasten with 4 screws (7) (Allen key size 8).
Inserting knives up to a length of 22 cm:
5. Insert the right cylindrical holder (4) from the inside outwards through
the right declination block (2) until the right locking pin (10) is released.
6. Pull cylindrical holder (4) outwards as far as it will go.
Put on cut-resistant safety gloves (see also chapter 8.1 ‘Additional
accessories’).
7. Insert the knife, from the center outwards, into the left cylindrical holder (3).
8. Slide the right cylindrical holder (4) onto the knife - support the knife from
below with one of your hands.
9. Slide knife guards (11) right and left over the knife edge.
10. Loosen Allen screw (8) on the left declination block.
11. Turn the knife forward until screws (9) are easily accessible.
12. Re-tighten Allen screw (8) on the left declination block (Allen key size 8).
13. Tighten the knife clamping screws (9) on both cylindrical holders with an
Allen key size 8 (alternately and only as many as the knife is wide).
14. Loosen Allen screw (8).
15. Turn the knife holder into desired clearance angle position (5), sustain it
in that position and tighten both Allen screws (8) (Allen key size 8).
5. Operation
Fig. 71
1
6
8
5
3
10
12
11
9
Fig. 72
2
7
8
4
9
10
11
12