Operation – Leica Biosystems SM2500 User Manual

Page 35

Leica SM2500

5.4.12 Area 4 - Section/milling stroke counter and thickness totalizer and reset buttons

42 CLEAR (button) - reset button for the section / milling stroke counter.

Pressing CLEAR resets counter (44) to ‘0’.

∑

∑ n Section / milling stroke counter.
Displays the total number of sections (or milling strokes) made since the
last reset.

43 CLEAR (button) - reset button for the section / milling thickness totalizer.

Pressing CLEAR resets counter (45) to ‘0’.

By resetting the totalizer (45) to ‘0’ you can define any level of the
specimen as ‘zero’ thickness (e.g. after a specimen has been
milled until it is exactly horizontal).
This feature gives you exact control on how much specimen mate-
rial (thickness) is removed from a defined point ‘zero’ onwards.
In addition, it enables you to go back to exactly the same vertical
knife / miller position after a manual upward movement (see area
5.1, chapter 5.4.13) of the knife / miller, i.e. after moving to a verti-
cal position of knife / miller < 0.

45 Display

∑

∑ μm - section / milling thickness totalizer.

Shows the total thickness of all sections / milling strokes carried out
since the last reset.

46 LED - indicates vertical position of knife/miller, relative to the selected

‘zero’ point, during manual movement (see area 5.1, chapter 5.4.13):
•

Knife / miller in vertical position > 0
= knife / miller moving from ‘zero’ downwards, towards the speci-
men surface: LED does not emit light.

•

Knife / miller in vertical position < 0
= knife / miller moving from ‘zero’ upwards, away from the speci-
men surface: LED emitting light.

Operation

Fig. 26

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Specimen

block

Knife

Vertical position < 0

Vertical position > 0

‘0’

Fig. 27