Leica Biosystems SM2500 - Manual (Page 59)

59 Leica SM2500
Inserting knives up to a length of 30 cm
Put on cut-resistant safety gloves (see also chapter 8.1 ‘Additional
accessories’).
Steps 1 through 4 - see previous page.
5. Insert the knife into the left cylindrical holder (3) guiding it through the
right declination block (2) from the outside inwards.
6 Slide the right cylindrical holder (4) from the outside inwards through the
right declination block (2) and onto the knife - support the knife from be-
low with one of your hands.
7. Slide knife guards (11) right and left over the knife edge.
8. Loosen Allen screw (8) on the left declination block (Allen key size 8).
9. Turn the knife forward until the knife clamping screws (9) are easily ac-
cessible.
10. Re-tighten Allen screw (8) on the left declination block (Allen key size 8).
11. Tighten the knife clamping screws (9) on both cylindrical holders with an
Allen key size 8 (alternately and only as many as the knife is wide).
12. Loosen Allen screw (8).
13. Turn the cylindrical holders into the desired clearance angle positon (5).
14. Sustain the knife holder in that position and tighten both Allen screws (8)
(Allen key size 8).
5.5.13 Inserting the disposable blade rail (only in knife holder B)
• Insert the blade rail into knife holder B as described for knives up to
22 cm length.
• Open the clamping screws on the blade rail (Allen key size 3).
• Insert the disposable blade; tighten the blade clamping screws, starting
from the center and working your way outwards from side to side.
5.5.14 Working with distance blocks for sectioning very high specimen blocks
• Place the distance blocks onto the bearing surfaces of the microtome,
then place the regular clamping blocks on top.
• Insert the extra-long fitting bolts through both blocks and tighten with an
Allen key, size 8.
5. Operation