Luminex IS Version 2.3 - Manual (Page 51)

x
MAP Technology Luminex 2.3 Software
PN 89-00002-00-110 Rev. B 41
During a backflush, the system draws sheath fluid from the sheath
fluid container and sends it directly to waste. You do not need to
supply solution in a plate. A backflush takes between 10 and 30
seconds.
‹
To clear obstructions from the cuvette:
Click Backflush, then click OK to verify that you want to
backflush the system.
The status bar shows that the backflush command is processing.
Run an Alcohol
Flush
Alcohol flush the system to remove air bubbles from the sample
tubing and the cuvette using 70% isopropanol or 70% ethanol. The
cuvette is the principal fluid pathway within the optics component of
the system where the system reads the sample.
‹
To remove air bubbles from the sample tubing and cuvette:
1. On the Maintenance tab, click Eject/Retract
2. A confirmation dialog box opens telling you to place solution in
the reservoir.
3. Put 70% isopropanol or 70% ethanol in the reservoir.
4. Click OK. The plate holder retracts, and the system performs the
Wash command.
Run a Wash Cycle Use the wash cycle after an alcohol flush or as needed. For example,
wash four times with distilled water after calibration and twice with
distilled water after Sanitize. Place at least 200
µ L in a microtiter
well or fill the Luminex XYP reservoir with distilled water. Washing
takes about 30 seconds.
You should wash after calibration and verification, between batches
and multi-batches, after sanitize, and before daily shutdown.
‹
To perform a Wash command:
1. On the Maintenance tab, click Eject/Retract.
2. Select Reservoir from the dropdown menu next to the Wash
button, then click Wash. A confirmation dialog box opens telling
you to place solution in the reservoir.
3. Put distilled water in the reservoir.
4. Click OK. The plate holder retracts, and the system performs the
Wash command.