Luminex FLEXMAP 3D Hardware User Manual - Manual (Page 54)

Symptom Possible cause Solution
The verification lot is expired. Use an unexpired bottle of
verification microspheres.
The verification microspheres
have been diluted. Do not dilute the verification
microspheres.
The sample probe height is
incorrect. Perform an automatic sample
probe height adjustment.
The sample probe is clogged. See the section titled “ Clogs ”.
There is air in the instrument. Verify the sample probe height.
Use the software to run a prime
command three times, an alcohol
flush command twice, then a wash
command three times with distilled
water.
The waste line was moved during
instrument operation, resulting in
an unstable flow rate. Stabilize the waste line during
instrument operation. See the
section titled “
Maintaining Fluids ”.
There is a problem internal to the
instrument. Review the log of calibration
reports. Check for dramatic
changes in temperature, sheath
pressure, or voltage. If any of
these are present, contact
Technical Support.
Acquisition Problems
TABLE 8. Verification Problems
Symptom Possible cause Solution
Acquisition fails or is slow. The air pressure is out of range. See the section titled
“
Pressurization Problems ”.
The sample probe height is
incorrect. Perform an automatic sample
probe height adjustment.
The sample probe is clogged. See the section titled “ Clogs ”.
The sheath or waste lines are not
fully connected. Disconnect and reconnect the
lines; you should hear them click.
The calibration microspheres have
expired. Replace old microspheres with an
unexpired lot.
The wrong wells are selected for
the calibration microspheres. Ensure that the correct wells are
selected in the software.
Troubleshooting
43