Luminex FLEXMAP 3D Hardware User Manual - Manual (Page 33)

Classification detector: Avalanche photo diodes with temperature compensation
Doublet discrimination detector: Avalanche photo diodes with temperature compensation
Fluidics
Cuvette: 200 micron square flow channel
Sample injection rate: 2 μL/second
Sample uptake volume: 20 to 200 μL
Sheath flow rate: 7.9 (+/- 0.9) mL/min
Sheath pressure: 8 to 13 psi for normal operations; 15 psi maximum
Microspheres
Distinguishes 1 to 500 unique xMAP microspheres in a single sample
Classification of xMAP microspheres: ≥ 80%
Total system misclassification of xMap microspheres: <= 4%
Internal sample carryover: < 1.5%
Detects a minimum of 500 fluorochromes of phycoerythrin (PE) per xMAP microsphere
Detects and distinguishes surface reporter fluorescence emissions at 575 nm on the surface of 1 to
500 unique xMAP microspheres in a single sample
Soluble background fluorescence emission at 575 nm automatically subtracted from fluorescence
intensity values.
Microtiter Plates
The Luminex ® FLEXMAP 3D ® system processes a 96-well microtiter plate in less than 20
minutes and a 384-well microtiter plate within 1 hour and 15 minutes, with 2500 microspheres
per region per well, counting 100 microspheres in each region.
Microtiter plates with 96 and 384 wells must be compatible with the microtiter heater block
temperature (from 35 to 60°C or 95° to 131°F) when the heater block is in use.
All microtiter plates (96 or 384 wells) have standard width (85.6 mm) and length (127.9 mm).
Depth varies depending on the type of well. To be compatible with the FLEXMAP 3D
instrument, the maximum allowable depth is 1”. Plates must have minimum 0.06” lip height,
standard distance from well center to well center (9 mm on 96-well plate), and standard
distance from A1 center to plate center in both length and width. To be compatible in size
with the microtiter heater block, the plate must fit into the heater block so that the top is flush
with the heater block.
FLEXMAP 3D ® Hardware User Manual
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