Luminex FLEXMAP 3D Hardware User Manual - Manual (Page 52)

Symptom Possible cause Solution
The microtiter plate is warped. Inspect the microtiter plate.
Replace it if it is warped.
The sample probe is bent. Remove the sample probe from
the instrument. Roll it on a flat
surface. If a sample probe has
been bent and rolled straight more
than once, discard it and replace it
with a new sample probe. Perform
an automatic sample probe height
adjustment using the software.
Calibration Problems
TABLE 6. Calibration Problems
Symptom Possible cause Solution
Calibration is slow or fails. The calibration microspheres are
not fully suspended. Vortex the calibration vials to
resuspend the microspheres.
The wrong lot number or target
values are entered in the
appropriate software user
interface. Check the lot number and target
values to make sure they are
correct.
The instrument calibrators are in
the wrong well. Verify that calibration
microspheres are in the correct
well.
There are not enough calibrator
microspheres in the well. Add at least five drops of calibrator
microspheres to the well. For
accurate drop volume, hold the
vial upside down at a 90 degree
angle to the microtiter plate while
dispensing them.
The calibrator lot is expired. Use an unexpired bottle of
calibrator microspheres.
The sample probe height is
incorrect. Perform an automatic sample
probe height adjustment.
The sample probe is clogged. See the section titled " Clogs ".
There is a partial clog in the
instrument. See the section titled " Clogs ".
Troubleshooting
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