Dako Autostainer - Manual (Page 7)

Table of Contents
Warnings, Precautions and Limitations............................................................................. 1
System Overview .............................................................................................................. 5
Software Overview ............................................................................................................ 7
Sign In Screen............................................................................................................... 7
Main Menu .................................................................................................................... 7
Initialize ......................................................................................................................... 8
Label Printing Initialization ........................................................................................ 8
Communication Port.................................................................................................. 9
Printer Selection...................................................................................................... 10
The Options Screen ................................................................................................ 10
Format Program Grid .............................................................................................. 10
IHC Report .............................................................................................................. 10
Custom Options ...................................................................................................... 10
Reagent Volume and Drop Zone Default ................................................................ 11
Clean........................................................................................................................... 11
Sign Off and Help........................................................................................................ 11
Reagent Tracking........................................................................................................ 11
Prime Pump ................................................................................................................ 12
Programming Grid Overview....................................................................................... 12
Entering Slide Information............................................................................................... 13
Adding Slides .............................................................................................................. 13
The Slide Info Button................................................................................................... 13
Deleting Slides ............................................................................................................ 14
Deleting Slides With the Slides Function................................................................. 14
Deleting Slide ID Information Using the Slide Info Button ....................................... 14
Rearranging slide positions on the Programming Grid ............................................... 14
Designing a Protocol ....................................................................................................... 15
Standard Protocol Elements ....................................................................................... 15
Additional Protocol Elements ...................................................................................... 16
Rinse Buffer ............................................................................................................ 16
Rinse Water ............................................................................................................ 16
Standard Rinse Replacement ................................................................................. 16
Substrate-Batch ...................................................................................................... 16
Switch...................................................................................................................... 16
Creating and Editing a Protocol Template .................................................................. 16
Deleting a Protocol Template...................................................................................... 17
Using a New Protocol Template Without Saving ........................................................ 17
Using a Saved Protocol Template............................................................................... 17
Using a Saved Protocol Template for a Specific Auto Program.................................. 18
Selecting Reagent Dispense Volume for All Steps in a Protocol ................................ 18
Selecting Reagent Dispense Volume for a Specific Step in a Protocol ...................... 18
Programming a Staining Run .......................................................................................... 19
Defining Detection Reagents ...................................................................................... 19
Defining Primary Antibodies........................................................................................ 20
Assigning Positive and Negative Control Reagents.................................................... 20
Adding Positive and Negative Control Reagents .................................................... 21
Autostainer Handbook vii