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Dako Autostainer User Manual

Page 34

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Assigning Reagent Dispense Locations


The non-frosted area of each slide is equally divided into three horizontal sections – or
dispense locations. Each slide can have one to three dispense locations, and the
maximum dispense volume of a slide cannot exceed 800 µL. The default dispense
location is programmed during initialization. Modifications to the dispense volume or
dispense location of a slide in the Slide Layout Map: Program Slides will change the
text color of that slide from black to red in the Slide Layout Map.

Printing a Slide Layout Map


The Slide Layout Map provides a record of reagent dispense volume and dispense
location. It is useful for ensuring that slides are loaded correctly.

1. Select the Next button on the Reagent Layout Map (see Section 8).
2. Select the Print button.

Selecting a new reagent-dispense location for all slides:


1. Select the All Slides tool in the upper left corner of the Slide Layout Map: Program

Slides.

2. Click the desired location on the slide to select a new reagent dispense location.
3. To assign multiple dispense locations on the All Slides tool, press and hold the

CTRL key while clicking on the desired dispense locations.

4. Click the dispense volume. A message displays the dispense volume choices.
5. Select the diamond next to the desired dispense volume.
6. Select the OK button or press ENTER.
7. The Slide Dispense Tool enables the user to make these changes for a specific

slide. The Slide Dispense Tool button tool functions identically to the All Slides tool
except the user is able to manipulate drop zone and reagent application amounts to
only the selected slide.

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Autostainer Handbook