Designing a protocol, Standard protocol elements – Dako Autostainer User Manual
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Section 5
Designing a Protocol
Protocol templates allow you to enter the series of steps required to stain a slide and
save the protocol for future use. Low level security does not allow creating or editing
protocol templates.
Standard Protocol Elements
Protocol elements are categories of reagents used to build the protocol outline in the
Protocol Template Design screen. Reagents can be added, deleted and edited by
choosing protocol element categories from the Edit Lists menu item on the
Programming Grid.
Endogenous Enzyme Block
Protein Block
Primary Antibody
Pretreatment
Secondary Reagent (e.g., biotinylated secondary antibodies or link)
Tertiary Reagent (e.g., enzyme-labeled streptavidin or label)
Labeled Polymer (e.g., used in the EnVision visualization systems)
Substrate-Batch
Substrate
Auxiliary (e.g., counterstain)
Autostainer Handbook
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