Dako Autostainer - Manual (Page 27)

Section 6
Programming a Staining Run
The Autostainer can process a maximum of 48 microscope slides in a single staining
run. Each slide can be stained using an independently designed protocol. After the slide
information is entered and protocol template is selected, specific reagents are assigned
to each slide. This is accomplished from the Programming Grid. The following section
describes how to program specific reagents for a staining run.
Each row
represents one
slide and its
information.
Reagents that
are available for
the highlighted
step.
Defining Detection Reagents
Before you can program specific reagents for slides, you must enter slide information
and select a protocol template as described in Section 4 and Section 5. The
Programming Grid is displayed listing the slides identified during Slide Count or Slide
Information entry. The first programmable field is highlighted and a list of available
reagents for the step is displayed.
1. Select a reagent using the UP and DOWN arrow keys and insert the reagent by
pressing ENTER. A message asks, “Assign to the following unprogrammed slides?”
2. Select Yes to assign the selected reagent to the remaining slides in the grid and the
next programmable step is highlighted. Select No and the reagent is assigned to only
the highlighted step.
3. After all reagents are programmed, click the Next button. The system checks the grid
for missing rinses. If a rinse is missing between two reagents, a message asks if you
want to continue.
Autostainer Handbook 19