Dako Autostainer - Manual (Page 39)

Section 8
Loading Reagents
After programming is complete, the Autostainer calculates the time required for the
staining run, the amount of wash buffer or deionized water required, and the most
efficient placement for the reagent vials.
Reagent Layout Map Screen
The Reagent Layout Map documents the rack positions of the reagents including the fill
volume needed for completing the staining run. It displays a maximum of 32 reagent
vials per rack. The abbreviations for the reagents are displayed in their appropriate
alpha-numeric rack positions.
Each reagent position is color coded to correspond to Dako reagent colors and the
colors from the Programming Grid. If more than 32 reagent vials are required, a
Second Rack button becomes available.
1. Select the Next button in the Slide Layout Map: Program Slides, a message asks,
“Save program on disk?”
2. Select No and the Reagent Layout Map appears.
3. Load the reagent vials according to the reagent map. The reagent volume displayed
includes a dead volume of at least 200 µL.
4. Make sure that enough reagent is in the vials to complete the programmed run and
that the reagent rack is properly seated in its correct location.
Autostainer Handbook 31