Dako Autostainer - Manual (Page 46)

Problem Possible Cause Course of Action
Buffer or DI Water does not flow
out during a run or a prime. (Cont.) Tubing is disconnected at
the inlet fitting(s). Check the tubing to verify
that the fittings are secure.
Inlet filters are clogged and
are restricting fluid flow. Check the inlet filter at the
end of the tubing in the
carboy - clean if necessary.
Dako recommends using a
tube and cap assembly
(S3472) to prevent foreign
objects from entering the
carboys and possibly
clogging the filter.
Tubing not completely
inserted into carboy.
Ensure that buffer and DI
water tubing is fully inserted
into the proper carboys.
Faulty buffer / DI water
pump. Contact a Dako
representative.
All waste collects into one waste
container. “Switch” is not programmed
in the Protocol Template. Program a “Switch” in the
Protocol Template.
Waste tubing not placed in
the proper waste carboy. Insure that all waste tubes
lead to proper carboy.
Faulty waste pump. Contact a Dako
representative.
Waste backs up in the sink. Clogged sink filter. Clean the filter when the
instrument is not in use.
Using a small brush and
some water, remove the
material causing the
blockage.
Reagent probe is not dispensing
reagent. Insufficient reagent in vial.
If the system detects
insufficient reagent to
complete the current step,
you have one minute to add
reagent, after which some
slides may be skipped.
Clogged reagent probe. Contact a Dako
representative.
Bubbles on the surface of
the reagent in the vial. Remove bubbles.
The yellow three-way
stopcock valve on the
syringe has been moved. Verify that the OFF position
of the valve is in the down
direction. The valve should
be in a “T” configuration.
Z-head assembly is hitting slide
racks during priming or during run. Arm is out of alignment. Emergency stop the run and
restart the run. Remove
steps already performed.
Recheck Reagent Layout
Map as this may cause vials
to be reassigned to new
locations.
Blow head/wash head is
bent. Contact a Dako
representative.
38 Autostainer Handbook