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Bio-Rad PROTEAN II XL Cell User Manual

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Section 11 Maintenance of Equipment ................................................................... 28

Section 12 Troubleshooting Guide - PAGE, SDS-PAGE, 2-D IEF/SDS-PAGE ........... 29

Section 13 Equipment and Accessories .................................................................. 31

13.1 PROTEAN II xi Cell - Slab Configurations .................................................. 31

13.2 Accessories ............................................................................................. 31

13.3 PROTEAN II xi Cell - 2-D Configuration .................................................... 34

13.4 Accessories ............................................................................................. 34

13.5 Electrophoresis Chemicals ....................................................................... 35

13.6 Power Supplies ........................................................................................ 36

Section 14 Appendix ................................................................................................ 37

14.1 Reagents and Gel Preparation for SDS-PAGE Slab Gels .......................... 37

14.2 Separating Gel Preparation ...................................................................... 38

14.3 Stacking Gel Preparation .......................................................................... 38

14.4 Running Conditions .................................................................................. 39

14.5 Comparison of Coomassie Blue and Silver Staining.................................. 39

14.6 2-D Stock Solutions ................................................................................. 39

14.7 Running Conditions .................................................................................. 40

Section 15 Bibliography ........................................................................................... 41

15.1 General

................................................................................................... 41

15.2 Native Gel Systems ................................................................................. 41

15.3 SDS Gel Systems .................................................................................... 41

15.4 Urea Gel Systems ................................................................................... 42

15.5 Two-Dimensional IEF / SDS-PAGE Gel Systems ...................................... 42

15.5 References

.............................................................................................. 42

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