Bio-Rad PROTEAN II XL Cell User Manual
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Section 11 Maintenance of Equipment ................................................................... 28
Section 12 Troubleshooting Guide - PAGE, SDS-PAGE, 2-D IEF/SDS-PAGE ........... 29
Section 13 Equipment and Accessories .................................................................. 31
13.1 PROTEAN II xi Cell - Slab Configurations .................................................. 31
13.2 Accessories ............................................................................................. 31
13.3 PROTEAN II xi Cell - 2-D Configuration .................................................... 34
13.4 Accessories ............................................................................................. 34
13.5 Electrophoresis Chemicals ....................................................................... 35
13.6 Power Supplies ........................................................................................ 36
Section 14 Appendix ................................................................................................ 37
14.1 Reagents and Gel Preparation for SDS-PAGE Slab Gels .......................... 37
14.2 Separating Gel Preparation ...................................................................... 38
14.3 Stacking Gel Preparation .......................................................................... 38
14.4 Running Conditions .................................................................................. 39
14.5 Comparison of Coomassie Blue and Silver Staining.................................. 39
14.6 2-D Stock Solutions ................................................................................. 39
14.7 Running Conditions .................................................................................. 40
Section 15 Bibliography ........................................................................................... 41
15.1 General
................................................................................................... 41
15.2 Native Gel Systems ................................................................................. 41
15.3 SDS Gel Systems .................................................................................... 41
15.4 Urea Gel Systems ................................................................................... 42
15.5 Two-Dimensional IEF / SDS-PAGE Gel Systems ...................................... 42
15.5 References
.............................................................................................. 42