Bio-Rad PROTEAN II XL Cell - Manual (Page 12)

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5. Level the casting stand on a flat surface with the alignment slot facing you. Check to see that gaskets are
clean and free of residual acrylamide to insure a good seal. Place a flat, grey, silicone gasket in each casting
slot.
Note: Always use the alignment slot to properly orient the gel sandwich. Failure to use this slot for alignment
can result in casting leaks while pouring the gel or buffer leaks during the run.
6. Place the assembled gel sandwich in the alignment slot of the casting stand. Loosen the clamp screws,
and allow the plates and spacers to align at the surface of the alignment slot. Insert a PROTEAN II xi
alignment card between the two glass plates to keep the spacers upright while additional alignment
adjustments are made. As an alternative, the alignment card can be positioned between the glass plates
when the sandwich is first assembled as in step 1.
7. Simultaneously push inward on both clamps at the locating arrows, and tighten both clamp screws just
enough to hold the sandwich in place. Pushing inward on both clamps at a point below the locating
arrows will insure that the spacers and glass plates are flush against the sides of the clamps.