Bio-Rad PROTEAN II XL Cell User Manual
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Table of Contents
Section 1 General Information ............................................................................... 1
1.1 Introduction .............................................................................................. 1
1.2 Specifications ........................................................................................... 1
1.3 Safety ...................................................................................................... 2
Section 2 Description of Major Parts ..................................................................... 3
2.1 Central Cooling Core ................................................................................ 3
2.2 Sandwich Clamps .................................................................................... 3
2.3 Casting Stand .......................................................................................... 3
2.4 Upper Buffer Chamber ............................................................................. 4
2.5 Lower Buffer Chamber ............................................................................. 4
2.6 Lid ........................................................................................................... 4
2.7 Tube Gel Adaptor ..................................................................................... 4
2.8 Alignment Card ........................................................................................ 4
Section 3 Assembling the Glass Plate Sandwiches.............................................. 4
3.1 Assembling Single Sandwiches ................................................................ 4
3.2 Assembling Multiple or “Double-up” Gel Sandwiches ............................... 8
Section 4 Casting the Gels ..................................................................................... 9
4.1 Casting Discontinuous (Laemmli) Gels ...................................................... 9
4.2 Casting Continuous Gels .......................................................................... 11
4.3 Casting Gradient Gels .............................................................................. 11
4.4 Casting Agarose Gels ............................................................................... 13
Section 5 Assembling the Upper Buffer Chamber ................................................ 15
5.1 Assembly ................................................................................................. 15
5.2 Use of the Buffer Dam .............................................................................. 17
Section 6 Loading the Samples ............................................................................. 18
6.1 Loading of Sample Wells .......................................................................... 18
6.2 Loading a Single Sample Per Gel ............................................................. 18
6.3 Gels as Samples ...................................................................................... 18
Section 7 Running the Gel...................................................................................... 19
Section 8 Set-up Options ....................................................................................... 19
8.1 Buffer Recirculation .................................................................................. 19
8.2 Cooling Options ....................................................................................... 20
Section 9 Removing the Gels ................................................................................. 21
Section 10 Two-Dimensional Electrophoresis ......................................................... 22
10.1 Sequence of Steps for 2-D Protocol ......................................................... 22
10.2 Protocol for IEF First Dimension................................................................ 22