Bio-Rad PROTEAN II XL Cell - Manual (Page 11)

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Section 3
Assembling the Glass Plate Sandwiches
3.1 Assembling Single Sandwiches
Note: Instructions for assembling 16 cm and 20 cm sandwiches are identical, except, of course, for the lengths
of the components. To insure proper alignment, make sure all plates and spacers are clean and dry before
assembly. The PROTEAN
® II xi plate washer/holder simplifies glass plate washing and also makes an ideal
storage system for clean, dry glass plates. Each plate holder will accommodate up to 8 PROTEAN II xi plates
or up to 18 Mini-PROTEAN
® II plates.
1. Assemble the gel sandwich on a clean surface. Lay the long rectangular plate down first, then place two
spacers of equal thickness along the long edges of the rectangular plate. Next, place a short plate on top
of the spacers so that it is flush with one end of the long plate.
2. Locate both the right and left sandwich clamps, and loosen the single screw of each by turning
counterclockwise. Place each clamp by the appropriate side of the glass plate stack, with the locating
arrows facing up and toward the glass plates.
3. Grasp the whole glass plate sandwich firmly with your right hand. With your left hand guide the left clamp
onto the sandwich so that the long and short plates fit the appropriate notches in the clamp. Tighten the
single screw enough to hold plates in place.
4. Place the right clamp on the right side of the plates, and tighten the clamp screw.