Bio-Rad PROTEAN II XL Cell - Manual (Page 20)

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7. Remove the comb very slowly to avoid tearing the gel.
Note: There are some special tricks to properly remove a comb from a vertical agarose gel. The most
important point is to introduce water or buffer under the comb while it is being removed. To introduce buffer
or water, use a squirt bottle or a needle and syringe to force fluid under the teeth of the comb while it is
slowly removed from the gel. Another option is to insert the comb only partway into the gel. This can easily
be done with the aid of comb conversion screws (catalog number 165-1859). The three standard screws on
the comb are replaced with the three large head comb conversion screws, with the protruding head of the
conversion screws resting on top of the longer outer glass plate. The well depth of the comb is limited to 10
mm instead of the standard 25 mm well depth.
A, Comb conversion screw B, Standard comb screw.