Bio-Rad PROTEAN II XL Cell - Manual (Page 13)

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8. Loosen one clamp screw slightly. While pushing down on the spacer with one finger, tighten the clamp
screw finger-tight with the other hand (see photo). This will insure proper sealing when solution is
poured. Tighten the other clamp screw in the same manner. It is important to visually inspect the
sandwich while it is in the alignment slot to insure that there are no gaps between the glass plates and
the surface of the alignment slot.
9. Remove the alignment card. Pull the gel sandwich from the alignment slot. Check that the plates and
spacers are flush at the bottom. If not, realign the gel sandwich as in steps 6-8.
Note: The easiest way to check for proper alignment is to run a fingernail across the contact area between
the glass plates and spacer. If your fingernail catches or drops as you move from plate to spacer to plate,
you must realign the sandwich before proceeding to step 10.