Bio-Rad PROTEAN II XL Cell - Manual (Page 14)

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10. The cams on the casting stand should be handle up and pulled out. Place the aligned sandwich into one
of the casting slots with the longer plate facing you (and the arrows on the clamp facing away from you).
When the sandwich is placed correctly, push the cams in, and turn them 180° so that the handles of the
cams point downward. The sandwiches are now ready for gel casting.
3.2 Assembling Multiple or “Double-up” Gel Sandwiches
Up to four gels can be run at one time by doubling up gel sandwiches (i.e., 2 gels/side). Double gels are
assembled, aligned, and cammed in a manner very similar to that described for single gels.
Note: In order to run four gels instead of two, it is necessary to order two notched inner glass plates and a
set of four spacers of equal length.
1. Lay down a long rectangular plate, two spacers, and a notched inner plate.
2. Place two more spacers on top of the notched inner plate.
Place the short inner glass plate on top of this set of spacers to form the complete double sandwich.
3. Apply the sandwich clamps as described in Section 3.1, steps 3-4. Insert two alignment cards between
each of the sandwiches to keep the spacers upright during sandwich alignment. Align, and then cam
the whole assembly into the casting stand. The sandwiches are now ready for gel casting.