Bio-Rad PROTEAN II XL Cell - Manual (Page 32)

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3. Attach a piece of silicone tubing to a 1 ml syringe and to the outside wall of the glass tubes on the top
end of the tube. Using the syringe filled with distilled water, apply a firm, even pressure to start the gel
extruding from the tubes. As the gel moves further out of the tube, apply less pressure so that rate of
extrusion remains constant. Care must also be taken not to extrude too quickly. Only slight pressure is
required to remove the last 1-2 cm of gel from the tube.
4. It is convenient to extrude the gels onto longitudinally folded pieces of Parafilm or plastic sheets.
5. Pipet 150 ml of transfer solution along the length of the extruded gel.