Eppendorf Temperature Verification System - Manual (Page 17)

Temperature Verification System USB – Single-channel — Operating manual
17
4.6.3 Preparing the Thermoblock 384
Prerequisites
The thermoblock with 384 wells is cooled to room temperature.
Fill the measuring positions with oil.
Inserting the spacers
Inserting the temperature sensor
 Insert the temperature sensor after the program started.
The position and the request to insert the sensor will be shown in the display.
The sensor cable will be guided outward over the thermoblock on the front of the
Mastercycler. It should be freely movable and should not be positioned near the lid closing
mechanism.
Hint!
Oil in the space between the temperature sensor and the thermoblock
improves the heat transmission. For measurements with the Temperature
Verification System USB – Single-channel, the bores are filled with 5 μL oil at
the specified measuring positions.
 Fill the bores with 5 μL oil at
the following positions:
•
F19
•
N22
•
E11
•
J14
•
C3
•
K6
 Insert the spacers before starting the
program.
The spacers will be inserted at the following
positions:
•
B5
•
B20
•
O5
•
O20
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
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