Ocean Optics pH Sensor Patches, Probes and Cuvettes User Manual
Page 18
2: Desktop Software Installation and Operation
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PHSENSOR-TRANS-REFLECT-201111
5. Enter your Experimental parameters: Acquisition Wavelength, Baseline Wavelength, and
approximate Ambient Temperature. Click Set, then click Next.
Note
For pH sensors that perform in the biological range (pH 5 – 9), the Acquisition Wavelength is
620nm and the Baseline Wavelength is referred to in the above specification tables for the
various form factors.
6. Enter the value for pK that came with your pH sensor. Then click Next.
7. Take a low pH reference spectrum at pH 1.0. To do this for your specific system, please refer to
the appropriate subsection below before moving on to 7a:
Transmissive pH Dip-Probe:
Submerge the probe tip in pH 1 buffer and shake back and forth to remove bubbles. Visually
check to ensure no bubbles are present in the optical path.
Reflective pH Patches:
Expose the patch to pH 1 buffer and ensure complete wetting of the mesh. Refer to the
previous section “Proper Sensor Wetting” for instructions on how to properly achieve this.
This is critical for successful calibration and sensor use.