Ocean Optics pH Sensor Patches, Probes and Cuvettes - Manual (Page 12)

1: Products
6 PHSENSOR-TRANS-REFLECT-201111
► Procedure
1. Peel the liner backing from the reflective pH sensor patch, and firmly adhere the patch to the
substrate with pressure. To avoid touching the mesh with bare fingers, use gloves or a Q-tip
to apply pressure to the center of the patch, ensuring there is total adhesive contact across the
patch.
2. Expose the sensor patch to pH 1 buffer or solution. Ensure total wetting of mesh material. See
proper sensor wetting.
3. Using a clamp or other securing apparatus, position the reflection probe flush against the wall
of the container, as perpendicular to the patch as possible. There should be no movement of
the probe or vessel; the sensory technique assumes unchanging optics.
4. Connect the illumination leg of the reflection probe to the light source.
5. Connect the collection leg of the reflection probe to the spectrometer.
6. Connect the spectrometer to the computer and warm up the light source as described in the
documentation for those products (see Product-Related Documentation ).
See Chapter 2: Desktop Software Installation and Operation for instructions on
calibration and taking pH measurements.
Proper Sensor Wetting
For the pH sensor patches to function properly and respond to environmental conditions, the mesh
material must be properly wetted before use. The best way to do this is with a dropper or pipette and a
visual check from the back side (probe side).
► Procedure
1. With the sensor submersed in pH 1 solution, use a dropper to create quick bursts of liquid
directly onto the patch; the dropper can be pushed against the patch without harming it. You
can visually see a darkening in the areas that have been wetted, versus a lighter appearance of
the still-dry sections.