Time acquisition experiments – Ocean Optics HR2000CG-UV-NIR User Manual
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Sample Experiments
HR2000 High-Resolution Fiber Optic Spectrometer
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Time Acquisition Experiments
OOIBase32 allows you to perform time acquisition experiments. Time acquisition experiments track processes,
perform kinetic analyses, and monitor spectral events all as a function of time. You can collect, as a function of
time, spectral data from up to six single wavelengths (designated as Channels A through F) and up to two
mathematical combinations of these wavelengths (designated as Combinations 1 and 2). Additionally, you can
acquire data in any mode (transmission, absorbance, etc.).
For more details about this and other OOIBase32 functions, refer to the
Follow the steps below to perform a time series experiment in OOIBase32:
1. Enter scope mode and store a reference spectra and dark spectra.
2. Choose the measurement mode (absorbance, transmission, etc.) and select Time Acquisition |
Configure | Configure Time Channels from the menu bar to access the Time Acquisition Channel
Configuration screen.
Proceed to the Configuring the Time Acquisition Channel Configuration Screen section below.
Configuring the Time Acquisition Channel Configuration Screen
1. Perform the following steps on the Time Acquisition Channel Configuration screen:
a. Select
Enabled to set the time acquisition calculation for the wavelength. The time acquisition
process will not calculate data if you do not select this option for at least
b. Select
Plotted to see a real-time graph of the acquired data in a spectral window.
c. Select
a
Spectrometer Channel for the time acquisition process
d. Specify the analysis wavelength in the Wavelength (nm) box.
e. Specify the number of pixels around the analysis wavelength to average in the Bandwidth
(pixels) box.
f. Select a multiplicative factor to apply to the data before plotting or storing. Then, select an
additive constant or offset to apply to the data. OOIBase32 applies the additive constant or offset
after applying the factor but before plotting or storing data.
The equation for the Factor and Offset functions is:
Results = (Factor * Data) + Offset
2. Configure a time acquisition process for the second single wavelength (if desired). Select the Channel B
page and repeat Steps 1-3 for Channel B.
To configure a time acquisition process for the third, fourth, fifth, and sixth single wavelengths, select the
Channel C, Channel D, Channel E, and Channel F pages, respectively, and set the necessary
parameters.
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