Ocean Optics HR2000CG-UV-NIR User Manual

Page 31

Sample Experiments

HR2000 High-Resolution Fiber Optic Spectrometer

Perform the following steps to take a relative irradiance measurement using OOIBase32:

1. Place OOIBase32 is in scope mode by clicking the Scope Mode icon on the toolbar, or by selecting

Spectrum | Scope Mode from the menu bar.

2. Ensure that the entire signal is on scale. The intensity of the reference signal should peak at about 3500

counts.

Note: The light source must be a blackbody of known color temperature.

3. In

the

Reference Color Temperature dialog box, enter the color temperature of the light source (in

Kelvin) and click the OK button.

4. Take a reference spectrum using a light source with a black body of a known color temperature, such as

the LS-1.

Click the Store Reference spectrum icon on the toolbar or select Spectrum | Store Reference from the
menu bar to store the reference. This command merely stores a reference spectrum in memory. You
must select File | Save | Reference from the menu bar to permanently save the spectrum to disk.

5. Block the light path to the spectrometer. Then, take a dark spectrum by clicking the Store Dark

Spectrum icon on the toolbar or by selecting Spectrum | Store Dark from the menu bar. This command
merely stores a dark spectrum in memory. You must select File | Save | Dark from the menu to
permanently save the spectrum to disk.

Note: If possible, do not turn off the light source when taking a dark spectrum. If you must turn off your

light source to store a dark spectrum, allow enough time for the lamp to warm up again before
continuing your experiment.

You must take a dark spectrum before measuring relative irradiance.

6. Position the fiber at the light source you wish to measure. Then, choose the Irradiance mode icon on the

toolbar or select Spectrum | Relative Irradiance Mode from the menu bar.

7. Click

the

Save icon on the toolbar or select File | Save | Processed from the menu bar to save the

spectrum to disk.

Note: If you change any sampling variable (integration time, averaging, smoothing, angle, temperature,

fiber size, etc.), you must store a new dark and reference spectrum.